斑节对虾TSG101基因的克隆及表达分析     

王芳  范嗣刚  赵超  王鹏飞  闫路路  邱丽华  闫喜武 《海洋科学》2019,43(7):122-131

为探究肿瘤易感基因101(简称TSG101)对斑节对虾(Penaeusmonodon)的免疫应答作用,了解在细菌刺激下斑节对虾的机体发生的变化机制,本研究以哈维弧菌(Vibrioharveyi)和金黄色葡萄球菌(Staphylococcusaureus)为实验组,以磷酸缓冲液(PBS)为对照组,通过荧光定量分析展开对斑节对虾对菌刺激的免疫应答作用。结果显示,斑节对虾的TSG101在各组织中均有表达,在肝胰腺中的表达量最高。在金黄色葡萄球菌刺激下,斑节对虾的TSG101在肝胰腺中的表达量与对照组相比呈极显著上调(P0.01),第12小时的TSG101 mRNA的表达量达到最大(为对照组的21.60倍);在鳃中的表达量与对照组相比呈极显著上调(P0.01),第6小时斑节对虾TSG101的表达量达到最大值(为对照组的3.64倍)。在注射哈维弧菌第9小时,肝胰腺中的PmTSG101 mRNA表达量极显著上调(P0.01)且达到最大(为对照组的2.50倍)。实验结果初步表明,斑节TSG101参与斑节对虾的先天免疫反应,在金黄色葡萄球菌和哈维弧菌的刺激的情况下,该基因RNA水平的表达情况发生明显变化。  相似文献   

新一代区域海-气-浪耦合台风预报系统   总被引：1，自引：0，他引：1  

雷小途  李永平  于润玲  李泓  汤杰  段自强  郑运霞  方平治  赵兵科  曾智华  黄伟  鲍旭炜  喻自凤  陈国民  马雷鸣  骆婧瑶  张帅  林立旻 《海洋学报》2019,41(6):123-134

依托国家重点基础研究（973）计划项目"上层海洋对台风的响应和调制机理研究"，中国气象局上海台风研究所联合国家海洋局的相关单位，通过实施近海台风的外场观测科学试验、加强台风边界层（特别是海气相互作用）物理过程诊断分析及参数化方案等的研究，建立并改进了台风强度预报的海-气-浪耦合预报模式系统，并在此基础上发展了台风强度的集合预报技术，在历史典型台风个例和2016-2017年台汛期的业务化测试中表现出良好的预报性能。  相似文献   

Test area of the SAGE survey     

Jie Zheng  Gang Zhao  Wei Wang  Zhou Fan  Ke-Feng Tan  Chun Li  Fang Zuo 《天文和天体物理学研究(英文版)》2019,(1)

Sky surveys represent one of the most important efforts to improve developments in astrophysics,especially when using new photometric bands. We are performing the Stellar Abundance and Galactic Evolution(SAGE) survey with a self-designed SAGE photometric system, which is composed of eight photometric bands. The project mainly aims to study the stellar atmospheric parameters of ～0.5 billion stars in ～12 000 deg2 of the northern sky, which mainly focuses on Galactic astronomy, as well as some aspects of extragalactic astronomy. This work introduces the detailed data reduction process of the test field NGC 6791, including the data reduction of single-exposure images and stacked multi-exposure images, and properties of the final catalog.  相似文献   

背负式移动激光扫描系统测绘大比例尺地形图精度试验研究   总被引：1，自引：1，他引：0  

谢宏全  陈岳涛  赵芳  田董炜  卢霞 《测绘通报》2019,(2):141-143,156

将背负式移动激光扫描系统应用在测绘大比例尺地形图中，其扫描精度至关重要。本文利用徕卡Pegasus Backpack对苏州工业园园区测绘地理信息大楼进行扫描，采用Inertial Explorer、Infinity、AoTumatic Processing对点云数据进行预处理，运用RealWorks提取特征点，将特征点在MicroStation V8联图中绘制成1：500地形图。通过与传统方法绘制的1：500地形图相叠合，发现两幅地形图具有很好重合度。对地物检测点精度分析后，得到点位中误差为0.026 m，高程中误差为0.041 m。研究结果表明徕卡Pegasus Backpack满足1：500地形图测量精度要求。  相似文献   

Multivariate adaptive regression splines for estimating riverine constituent concentrations     

Hong Huang  Xiaoliang Ji  Fang Xia  Shuhui Huang  Xu Shang  Han Chen  Minghua Zhang  Randy A. Dahlgren  Kun Mei 《水文研究》2020,34(5):1213-1227

Regression-based methods are commonly used for riverine constituent concentration/flux estimation, which is essential for guiding water quality protection practices and environmental decision making. This paper developed a multivariate adaptive regression splines model for estimating riverine constituent concentrations (MARS-EC). The process, interpretability and flexibility of the MARS-EC modelling approach, was demonstrated for total nitrogen in the Patuxent River, a major river input to Chesapeake Bay. Model accuracy and uncertainty of the MARS-EC approach was further analysed using nitrate plus nitrite datasets from eight tributary rivers to Chesapeake Bay. Results showed that the MARS-EC approach integrated the advantages of both parametric and nonparametric regression methods, and model accuracy was demonstrated to be superior to the traditionally used ESTIMATOR model. MARS-EC is flexible and allows consideration of auxiliary variables; the variables and interactions can be selected automatically. MARS-EC does not constrain concentration-predictor curves to be constant but rather is able to identify shifts in these curves from mathematical expressions and visual graphics. The MARS-EC approach provides an effective and complementary tool along with existing approaches for estimating riverine constituent concentrations.  相似文献   

The carotenoid characteristics of the important wild shrimp Trachysalambria curvirostris(Stimpson,1860) in China     

SU Fang  LIU Jianguo 《中国海洋湖沼学报》2019,(2)

Trachysalambria curvirostris is an economically important shrimp in the coastal waters of the Western Indo-Pacific. However, there is no information about its carotenoid composition and distribution.The carotenoid profiles including concentration, composition, molecular forms(free or esterified) and configuration(geometrical and optical isomers) of raw and dried T. curvirostris(Stimpson, 1860) were qualitatively and quantitatively assessed. In the raw shrimp, astaxanthin and β-carotene were the two dominant carotenoids, which contributed 75.18%–88.86% and 2.86%–16.83% of the total carotenoid content. Traces of echinenone, canthaxanthin and astacene were also detected. The carotenoid content in the waste(80.28 mg/kg dry weight) was nearly two-fold higher than that in the meat(49.69 mg/kg dry weight). Of the total astaxanthin in these shrimp, 85.18%–90.59% was esterified. All-trans astaxanthin was the predominant geometrical isomer followed by 13-cis, 9-cis, di-cis and 15-cis isomers. A mix of three stereoisomers was found in all parts and the percentages of meso(3 S, 3' R) and(3 S, 3' S) forms were significantly higher than the(3 R, 3' R) isomer. Compared to the carotenoid content of the raw shrimp,only 26.17 mg/kg remained in the dried shrimp. Nearly half of the carotenoids were lost during the drying process. Substantial hydrolysis and isomerization also occurred.  相似文献   

Assessment of Surface Ecological Quality of Grassland Mining Area and Identification of Its Impact Range     

Fang  Aman  Bao  Menghe  Chen  Weiqiang  Dong  Jihong 《Natural Resources Research》2021,30(5):3819-3837

Natural Resources Research - High-intensity coal mining in grasslands has negative impact on the surface ecology of the mining area and its surroundings. This impact shows spatio-temporal...  相似文献   

祁连山疏勒河源区冻土退化对土壤微生物生物量碳氮的影响   总被引：2，自引：1，他引：1  

吴明辉  瞿德业  李婷  刘放  高雅月  陈生云  陈拓 《地理科学》2021,41(1):177-186

对青藏高原东北缘祁连山西段疏勒河源区多年冻土区0~50 cm土壤微生物生物量碳氮分布特征及其影响因素进行分析。结果表明:稳定型和极不稳定型多年冻土区0~50 cm土壤中微生物量碳含量范围分别为0.015~0.620 g/kg和0.019~0.411 g/kg,微生物量氮含量范围分别为0.644~12.770 mg/kg和0.207~3.725 mg/kg;土壤微生物量总体呈现出稳定型显著高于极不稳定型多年冻土,表明多年冻土退化（多年冻土由稳定型退化为极不稳定型）对土壤微生物量积累有明显抑制作用。土壤微生物生物量碳占有机碳、微生物生物量氮占全氮的比值在稳定型多年冻土中显著高于极不稳定型,表明多年冻土退化对土壤微生物的矿化能力有明显抑制作用。土壤微生物量及其与土壤养分的比值有显著的剖面变化特征,随土壤深度增加而减小。土壤微生物量碳氮均与土壤温度显著负相关,与地下生物量显著正相关。稳定型多年冻土中,土壤微生物量碳氮与碳氮比正相关、与氧化还原电位负相关;不稳定型多年冻土中,土壤微生物量碳氮与pH正相关。土壤微生物量碳氮与土壤温度和pH在剖面变化上显著相关。逐步回归分析表明驱动微生物生物量碳氮在不同多年冻土类型和土层之间变化的因子是不同的。  相似文献   

湖南省传统农区乡村功能时空演变及影响因素研究   总被引：2，自引：1，他引：1  

谭雪兰  蒋凌霄  安悦  余航菱  周舟  李印齐 《地理科学》2021,41(12):2168-2178

乡村是城市功能扩散与转移的重要承接地,乡村功能的时空演变及影响因素分析对实现城乡统筹发展与可持续发展具有重要意义。综合运用熵值法、冷热点分析和地理探测器等方法,以湖南省102个县市为研究对象,对湖南省乡村功能的时空演变、空间分异及其影响因素进行研究。结果表明：① 1997―2017年县域乡村生产和生活功能整体上呈现出上升的发展态势,而乡村生态功能整体上呈减弱的趋势。② 1997―2017年湖南省乡村功能热点区由长株潭地区向外扩张,而冷点区呈先收缩后扩张的发展态势;20 a间,在长株潭地区形成稳定的热点区,在湘西地区形成稳定的冷点区。③ 湖南省乡村功能空间分异是农业现代化、乡村就业主体、县域经济基础、城镇化、工业化等综合作用的结果,其影响力大小与方向存在显著的差异性,其中农业现代化水平、乡村就业主体对湖南省及5个地区乡村功能空间分异有重要的驱动作用。  相似文献   

文化消费者对黄梅戏展演空间感知的模式与价值寻求：方法−目的链理论的应用     

朱芳  苏勤  陶云 《地理科学》2021,41(10):1822-1831

以安庆黄梅戏会馆和再芬黄梅公馆为例,基于方法?目的链理论模型,解读文化消费者对黄梅戏展演空间属性的感知评价、层次处理和价值导向的转变。研究发现：不同层次的空间属性在文化消费者的感知与体验中实现着新的组合与重构,由此形成了基于舞台展演内容、文化呈现形式以及演出环境氛围为主体的3种空间感知模式。结合文化消费者感知的心理结构与路径,黄梅戏展演空间被消费者建构为娱乐空间、情感空间和社交空间,空间的多元化意义和价值得到彰显。方法?目的链理论从消费者的感知与需求出发,探索文化空间意义生成路径,实现了文化空间与消费者之间的双向信息交换。  相似文献